Increased cerebrospinal fluid adenosine 5'-triphosphate in patients with amyotrophic lateral sclerosis

Abstract Background Extracellular adenosine 5'-triphosphate (ATP) has been suggested to cause neuroinflammation and motor neuron degeneration by activating microglia astrocytes in amyotrophic lateral sclerosis (ALS). Since we have developed a highly sensitive ATP assay system, examined cerebrospinal fluid (CSF) levels patients with ALS whether it can be useful biomarker ALS. Methods Forty-eight CSF samples from 44 were assayed for newly established, system using luciferase luminous reaction. idiopathic normal pressure hydrocephalus (iNPH) as control. Patients divided into two groups depending on their disease severity, evaluated the Medical Research Council (MRC) sum score. Correlations between other factors, including clinical data serum creatinine levels, evaluated. Results significantly higher than iNPH (716 ± 411 vs. 3635 5465 pmol/L, p < 0.01). more severe group (6860 8312 716 0.05) mild 2676 3959 respectively. functional rating scale-revised (ALSFRS-R) (37.9 5.7 42.4 2.8, 0.01) (0.51 0.13 0.68 0.23 mg/dL, lower A negative correlation of MRC score was demonstrated analysis adjusted age sex (r = -0.3, 0.08). Conclusions is particularly increased advanced may evaluating severity